Absorptive Lipid Calculator

Step 1 Lipid mixture (alone) Blank absorbance of the lipid carrier, measured without RNA.

Lipid

Pre-formulation

RNA input (ng/µL, pre-formulation)

Input lipid conc. (mM, pre-formulation)

Ionizable lipid (mol% of mix)

Parameters

Formulation ratio (aqueous : organic)

RNA type (sets nucleotide MW)

Nucleotide MW (g/mol per phosphate)

Factors & results

Absorption factor (ng/µL per mM lipid in the 1:1 sample)

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Negative concentration compensation (ng/µL, subtracted from each 1:1 reading)

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N:P ratio (calculated)

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Step 2 RNA samples Enter measured RNA reading (ng/µL) per sample; replicates averaged.

Dilution fixed at 1:1 (×2) · measure every sample at a 1:1 dilution for accurate reading using this calculator.

Sample	Conc. 1	Conc. 2	Conc. 3	Compensated	Final

Preview Compensated result per sample

Measured (raw mean) Compensated